lanes 1 (New England Biolabs)

New England Biolabs is a verified supplier

New England Biolabs manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

New England Biolabs lanes 1

Molecular characterization <t>of</t> <t>T-DNA</t> integration and Cas9 expression in transformants. (A) PCR-based screening of the primary transformants. Lane M: 100 bp DNA ladder (Thermo Fisher Scientific); lanes 1–93: DNA of the recovered plants; lane WT: wild-type; lane NTC: No template control (water); lane PC: positive control (pHSE401 vector). (B) PCR analysis of the hygromycin-resistant T 1 plants. Lane M: 1 kb plus DNA ladder (NEB); lanes <t>1–15:</t> DNA of the hygromycin-resistant T 1 plants; lane wt: wild-type; lane NTC: No template control; lane pc: positive control. (C) Relative expression of the Cas9 gene in T 0 (i) and T 1 (ii) transformants determined by RT-qPCR. Gene expression levels were normalized using the 2 ⁻ΔΔCt method, with GhGAPDH as the internal reference gene and the wild-type sample as the calibrator. Error bars represent the standard error (SE) of three replicates.

Lanes 1, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 565 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lane+1/1+kb+Plus+DNA+Ladder/pmc13094215-245-8-7
Average 98 stars, based on 565 article reviews

lanes 1 - by Bioz Stars, 2026-09

98/100 stars

Images

1) Product Images from "CRISPR/Cas9-mediated editing of the GhJAZ2 gene improves fiber length and lint percentage in Gossypium hirsutum L"

Article Title: CRISPR/Cas9-mediated editing of the GhJAZ2 gene improves fiber length and lint percentage in Gossypium hirsutum L

Journal: GM Crops & Food

doi: 10.1080/21645698.2026.2660546

Molecular characterization of T-DNA integration and Cas9 expression in transformants. (A) PCR-based screening of the primary transformants. Lane M: 100 bp DNA ladder (Thermo Fisher Scientific); lanes 1–93: DNA of the recovered plants; lane WT: wild-type; lane NTC: No template control (water); lane PC: positive control (pHSE401 vector). (B) PCR analysis of the hygromycin-resistant T 1 plants. Lane M: 1 kb plus DNA ladder (NEB); lanes 1–15: DNA of the hygromycin-resistant T 1 plants; lane wt: wild-type; lane NTC: No template control; lane pc: positive control. (C) Relative expression of the Cas9 gene in T 0 (i) and T 1 (ii) transformants determined by RT-qPCR. Gene expression levels were normalized using the 2 ⁻ΔΔCt method, with GhGAPDH as the internal reference gene and the wild-type sample as the calibrator. Error bars represent the standard error (SE) of three replicates.

Figure Legend Snippet: Molecular characterization of T-DNA integration and Cas9 expression in transformants. (A) PCR-based screening of the primary transformants. Lane M: 100 bp DNA ladder (Thermo Fisher Scientific); lanes 1–93: DNA of the recovered plants; lane WT: wild-type; lane NTC: No template control (water); lane PC: positive control (pHSE401 vector). (B) PCR analysis of the hygromycin-resistant T 1 plants. Lane M: 1 kb plus DNA ladder (NEB); lanes 1–15: DNA of the hygromycin-resistant T 1 plants; lane wt: wild-type; lane NTC: No template control; lane pc: positive control. (C) Relative expression of the Cas9 gene in T 0 (i) and T 1 (ii) transformants determined by RT-qPCR. Gene expression levels were normalized using the 2 ⁻ΔΔCt method, with GhGAPDH as the internal reference gene and the wild-type sample as the calibrator. Error bars represent the standard error (SE) of three replicates.

Techniques Used: Expressing, Control, Positive Control, Plasmid Preparation, Quantitative RT-PCR, Gene Expression

Polyacrylamide Gel Electrophoresis:

Article Title: Endogenous Glutathione-Activated Nucleic Acid Molecular Circuitry for Cell-Specific MicroRNA Imaging
Article Snippet: .. Inset: Denatured PAGE analysis of GSH-mediated H2-P cleavage at 37 °C for 5 h (1 × NEBuffer 4 buffer), lane 1, H2-P (400 nM), 0 mM, lane 2, cleaved product H2, lane 3, 3 mM, lane 4, 5 mM, lane 5, 8 mM, lane 6, 10 mM, lane 7, 12 mM, lane 8, 15 mM. (D) Optimization of the molar ratio between hairpins H1 and H2-P with (orange bars) or without (grey bars) miR-21. ..

Infection:

Article Title: <i>Epichlöe festucae</i> endophyte mediated maternal inheritance of dollar spot disease resistance in hard fescue
Article Snippet: Funding information Rutgers Center for Turfgrass Science; Northwest Turfgrass Association; Oregon State University Agricultural Research Foundation Abstract Hard fescue (Festuca brevipila) is a fine-leaved cool-season turfgrass and is well adapted for low-maintenance areas, such as home lawns, parks, and roadsides.. Breeding for improved disease resistance is a major objective in utilizing hard fescue under low-input management.. The Epichlöe festucae endophyte-mediated dollar spot (Clarireedia jacksonii) resistance and its mechanism have been well-documented in strong creeping red fescue (F. rubra subsp. rubra).

Plasmid Preparation:

Article Title: Influence of mobile genetic elements and insertion sequences in long- and short-term adaptive processes of Acidithiobacillus ferrooxidans strains.
Article Snippet: .. Lane M represents a 1-kb DNA ladder; lane 1 represents uncut plasmid DNA; lane 2 represents plasmid DNA digested with a BamHI-HF® restriction endonuclease (New England Biolabs) for 1 h at 37 °C. ..

Article Title: Influence of mobile genetic elements and insertion sequences in long- and short-term adaptive processes of Acidithiobacillus ferrooxidans strains
Article Snippet: .. Lane M represents a 1-kb DNA ladder; lane 1 represents uncut plasmid DNA; lane 2 represents plasmid DNA digested with a BamHI-HF® restriction endonuclease (New England Biolabs) for 1 h at 37 °C. ..

Molecular Weight:

Article Title: Isolation and characterization of ɸEcM-vB1 bacteriophage targeting multidrug-resistant Escherichia coli
Article Snippet: .. Lane 1 shows the XLarge DNA ladder (Gene DireX) and lane 2 shows a band of phage DNA with a size of approximately 30- 40 kb, B: Lane 1 shows a 1 kb DNA ladder (New England Biolabs) and lane 2 shows the EcM-vB1 phage DNA restriction analysis with EcoR1; C: Image shows the SDS‒PAGE analysis of the EcM-vB1 phage structural proteins; lane 1 shows broad range protein molecular weight markers (The Novex™ sharp prestained protein standard, Life Technologies) and lane 2 shows the EcM-vB1 phage proteins. .. Lane 1 shows the XLarge DNA ladder (Gene DireX) and lane 2 shows a band of phage DNA with a size of approximately 30- 40 kb, B: Lane 1 shows a 1 kb DNA ladder (New England Biolabs) and lane 2 shows the EcM-vB1 phage DNA restriction analysis with EcoR1; C: Image shows the SDS‒PAGE analysis of the EcM-vB1 phage structural proteins; lane 1 shows broad range protein molecular weight markers (The NovexTM sharp prestained protein standard, Life Technologies) and lane 2 shows the EcM-vB1 phage proteins.

Electroporation:

Article Title: Transforming the untransformable with knockout minicircles: High-efficiency transformation and vector-free allelic exchange knockout in the fish pathogen Photobacterium damselae.
Article Snippet: .. Washed cells are typically resuspended in an electroporation solution containing glycerol that prevents freezing damage and F IGURE 1 aip56 knockout (KO) minicircle: (a) Amplicons of the assembly fragments: Lane 1—Fast DNA Ladder (NEB) marker with 10, 5, 3, 2, 1.5, 1 (reference), 0.766, 0.5, 0.3, 0.15, and 0.05 kb bands; Lane 2—kanR2 gene sequence (Km_F/R); Lane 3—sacB transcript sequence (sacB_F/ R); Lane 4—aip56 upstream homology arm (aip56_up_F/R); Lane 5—aip56 downstream homology arm (aip56_down_F/R). (b) Schematic representation of the assembled minicircle. ..

Knock-Out:

Article Title: Transforming the untransformable with knockout minicircles: High-efficiency transformation and vector-free allelic exchange knockout in the fish pathogen Photobacterium damselae.
Article Snippet: .. F IGURE 2 ssrA/smpB knockout (KO) minicircle: (a) Amplicons of the assembly fragments: Lane 1—Fast DNA Ladder (NEB), marker with 10, 5, 3, 2, 1.5, 1 (reference), 0.766, 0.5, 0.3, 0.15, and 0.05 kb bands; Lane 2—kanR2 gene sequence (Km_F/R); Lane 3—sacB transcript sequence (sacB_F/R); Lane 4—ssrA/smpB upstream homology arm (ssrA_up_F/R); Lane 5—ssrA/smpB downstream homology arm; (ssrA_down_F/R). (b) Schematic representation of the assembled minicircle. ..

Marker:

Article Title: Transforming the untransformable with knockout minicircles: High-efficiency transformation and vector-free allelic exchange knockout in the fish pathogen Photobacterium damselae.
Article Snippet: .. F IGURE 2 ssrA/smpB knockout (KO) minicircle: (a) Amplicons of the assembly fragments: Lane 1—Fast DNA Ladder (NEB), marker with 10, 5, 3, 2, 1.5, 1 (reference), 0.766, 0.5, 0.3, 0.15, and 0.05 kb bands; Lane 2—kanR2 gene sequence (Km_F/R); Lane 3—sacB transcript sequence (sacB_F/R); Lane 4—ssrA/smpB upstream homology arm (ssrA_up_F/R); Lane 5—ssrA/smpB downstream homology arm; (ssrA_down_F/R). (b) Schematic representation of the assembled minicircle. ..

Article Title: The mismatch repair endonuclease MutLα tethers duplex regions of DNA together and relieves DNA torsional tension.
Article Snippet: .. Lane 1 contains 1 kb plus DNA ladder (NEB), lane 2 contains a 4.3 kb DNA marker and lane contains DNA inputs for 2.7 kb (135 ng, 77 fmol) plasmids. ..

Sequencing:

Article Title: Transforming the untransformable with knockout minicircles: High-efficiency transformation and vector-free allelic exchange knockout in the fish pathogen Photobacterium damselae.
Article Snippet: .. F IGURE 2 ssrA/smpB knockout (KO) minicircle: (a) Amplicons of the assembly fragments: Lane 1—Fast DNA Ladder (NEB), marker with 10, 5, 3, 2, 1.5, 1 (reference), 0.766, 0.5, 0.3, 0.15, and 0.05 kb bands; Lane 2—kanR2 gene sequence (Km_F/R); Lane 3—sacB transcript sequence (sacB_F/R); Lane 4—ssrA/smpB upstream homology arm (ssrA_up_F/R); Lane 5—ssrA/smpB downstream homology arm; (ssrA_down_F/R). (b) Schematic representation of the assembled minicircle. ..

Review